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Title: Targeted expression of genes for manipulation of the systemic acquired resistance responses of cotton for improved tolerance to fungal pathogens
Authors: McFadden, Helen
Keywords: targeting transgene expression in plants
expression of transgenes
Ac element
Ac. Transposition
transposable element
limiting gene function
target gene
PR10 gene family
Issue Date: 30-Jun-2003
Publisher: CSIRO Plant Industry
Series/Report no.: ;CSP115C
Abstract: The overall aim of this project was to develop systems for targeting transgene expression in plants to enhance their defence responses. We were investigating two methods for the regulation of the expression of transgenes in transgenic cotton. One method involved limiting gene function by insertion of the transposable element Ac. Transposition of the Ac element (where the Ac element “jumps out” of the gene) can cause localised re-activation of the target gene. This could be used to give small areas of localised gene expression in instances where constitutive expression of the gene in all cells would have a detrimental effect. This could be useful in the case of avirulence genes, for example. The use of transposable elements has other potential uses for gene tagging and we wished to demonstrate its feasibility in cotton. The second method involved an investigation of the behaviour of some potentially pathogen-inducible promoters as drivers of transgene expression. In this study our focus was on the PR10 gene family. Members of this gene family are highly induced by Fusarium infection of cotton – in fact they constitute a significant component of cotton’s responses to Fusarium.
Appears in Collections:2003 Final Reports

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