The results of this study have revealed that the number of freshwater fishes (species diversity) of the King Edward River is higher than has previously been recorded for a Western Australian river. Twenty-six freshwater fish species were recorded, which is three species higher than the much larger Fitzroy River in the southern Kimberley. The study also resulted in a number of range extensions, including Butler's Grunter and Silver Cobbler to the west, and the Slender Gudgeon to the north and east. What appears to be an undescribed species of glassfish was captured. It differs morphologically from described species in head spines (or lack of), fin rays, as well as relative body measurements. Similarly, an undescribed ectoparasite, Argulus sp. (Crustacea: Branchiura), was found on the caudal (tail) fin lobes of one Black Bream (Jenkin's Grunter) and three Spangled Perch. Interestingly, a considerable proportion of Black Bream, which is widespread throughout the system but essentially restricted to main channel sites, had 'blubber-lips'.