Fusarium wilt is a destructive disease of cotton which occurs in many cotton growing areas of the world, including; south-eastern USA, Egypt, Tanzania and China. It is caused by the soil inhabiting Fusarium oxysporum f.sp vasinfectum (Fov) (Hillocks, 1992) The first confirmed record of the disease in Australia was from wilted cotton collected from the Brookstead/Cecil Plains area in the Darling Downs of Queensland, in March 1993 (Kochman, 1995). Fusarium wilt has since been recorded in many commercial cotton crops on the Downs and in isolated locations at Mungindi, Boggabilla, Goondiwindi and Moree. More recently, the disease was confirmed in wilting plants in the Theodore and Miles areas of Queensland. To date, Fusarium wilt has not been recorded in the Emerald or St George areas of Queensland or cotton production areas in the Namoi and Macquarie Valleys of New South Wales