A survey was conducted to ascertain the presence of feral cotton plants in off-farm drainage areas and along roadsides associated with cotton production and transport throughout Queensland and parts of northern NSW. The establishment of these plants is the direct result of the movement of cotton seed from overland flow associated with irrigation runoff into common drainage lines or module road freight. Perennial plants were sub-sampled to determine disease status, transgenic heritage, and pest insect presence as well as geotagged for future reference.