Resistance to poses a threat to the continued sustainable production of cotton that transgenic agriculture has enabled. This project provided preliminary genomic data to investigate the evolution of resistance to glyphosate in agricultural weeds. With the Science award funding I sequenced the transcriptome (expressed genes) and also part of the genome of two weed species that have recently developed resistance to glyphosate; Fleabane (Conyza bonariensis) and Awnless Barnyardgrass (Echinochloa colona). These data form the basis of a post-doc project exploring the role of genetic diversity in the evolution of resistance, and methods that use genomic approaches to identify novel resistance mechanisms.